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| Registros recuperados : 17 | |
| 6. |  | NUNES, P. S. de O.; JÚNIOR, G. V. L.; MASCARIN, G. M.; GUIMARÃES, R. A.; MEDEIROS, F. H. V. de; ARTHURS, S.; BETTIOL, W. Microbial consortia of biological products: do they have a future? Biological Control, v. 188, article 105439, 2024.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | TALAMINI, V.; CARVALHO, E. de A.; MEDEIROS, F. H. V. de; PEREIRA, P. de F.; OLIVEIRA, F. A. de; SANTOS, C. C. dos; SILVA, J. C. da. Prospecção e eficiência de antagonistas à resinose do coqueiro (Thielaviopsis paradoxa) nos Tabuleiros Costeiros e na Amazônia Oriental. In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3.; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS, 3., 2014, Aracaju. Seifrut: [anais..]. Brasília, DF: Embrapa, 2014. p. 172-176. Editores Técnicos: Carlos Roberto Martins, Francisco Alisson Xavier, Antônio Souza do Nascimento.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Tabuleiros Costeiros. |
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| 8. | | BUENO, A. de F.; CARVALHO. G. A.; NOGUEIRA, M. A.; MEDEIROS, F. H. V. de; MEDEIROS, F. C. L. de; HUNGRIA, M.; ARDISSON-ARAÚJO, D. M.; RIBEIRO, B. M.; SÓSA-GOMEZ, D. R.; HIROSE, E. Compatibilidade no uso de bioinsumos e insumos sintéticos no manejo da cultura da soja. In: MEYER, M. C.; BUENO, A. de F.; MAZARO, S. M.; SILVA, J. C. da (ed.). Bioinsumos na cultura da soja. Brasília, DF: Embrapa, 2022. cap. 27. p. 473-492.| Biblioteca(s): Embrapa Soja. |
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| 9. | | MARTINS, S. A.; SCHURT, D. A.; SEABRA, S. S.; MARTINS, S. J.; RAMALHO, M. A. P.; MOREIRA, F. M. de S.; SILVA, J. C. P. da; SILVA, J. A. G. da; MEDEIROS, F. H. V. de. Common bean (Phaseolus vulgaris L.) growth promotion and biocontrol by rhizobacteria under Rhizoctonia solani suppressive and conducive soils. Applied Soil Ecology, 2017.| Biblioteca(s): Embrapa Roraima. |
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| 10. | | REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W. Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum. World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | GUIMARÃES, R. A.; ZANOTTO, E.; PERRONY, P. E. P.; ZANOTTO, L. A. S.; SILVA, L. J. da; MACHADO, J. da C.; PINTO, F. A. M. F.; MEDEIROS, H. N.; Von PINHO, R. G.; MELO, I. S. de; SILVA, J. C. P. da; MEDEIROS, F. C. L. de; MEDEIROS, F. H. V. de. Integrating a chemical fungicide and Bacillus subtilis BIOUFLA2 ensures leaf protection and reduces ear rot (Fusarium verticillioides) and fumonisin content in maize. Journal of Phytopathology, v. 169, n. 3, p. 139-148, 2020.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | PINTO, F. A. M. F.; PORTO, V. B. C.; GUIMARÃES, R. A.; SIQUEIRA, C. da S.; FARIA, M. R. de; MACHADO, J. da C.; MEDEIROS, H. N.; SILVA, D. D. da; SANTOS NETO, H.; POZZA, E. A.; MEDEIROS, F. H. V. de. Detection and factors that induce Stenocarpella spp. survival in maize stubble and soil suppressiveness under tropical conditions. Applied Sciences, v. 12, 4974, 2022.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 13. | | MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; OLIVEIRA, M. C. N. de; JACCOUD FILHO, D. de S.; VENANCIO, W. S.; MEDEIROS, F. H. V. de; JULIATTI, F. C.; CARNEIRO, L. C.; NUNES JUNIOR, J.; MARTINS, M. C. Experimentos cooperativos de controle biológico de Sclerotinia sclerotiorum na cultura da soja: Resultados sumarizados da safra 2018/2019. Londrina: Embrapa Soja, 2019. 5 p. (Embrapa Soja. Circular técnica, 155).| Biblioteca(s): Embrapa Soja. |
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| 14. | | MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; OLIVEIRA, M. C. N. de; NUNES JUNIOR, J.; LOBO JUNIOR, M.; JACCOUD FILHO, D. de S.; VENANCIO, W. S.; MEDEIROS, F. H. V. de; JULIATTI, F. C.; CARNEIRO, L. C.; BRUSTOLIN, R. Experimentos cooperativos de controle biológico de Sclerotinia sclerotiorum na cultura da soja: resultados sumarizados da safra 2019/2020. Londrina: Embrapa Soja, 2020. 19 p. (Embrapa Soja. Circular técnica, 163).| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Soja. |
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| 15. | | MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; OLIVEIRA, M. C. N. de; VENANCIO, W. S.; BRUSTOLIN, R.; CARNEIRO, L. C.; NUNES JUNIOR, J.; LOBO JUNIOR, M.; JULIATTI, F. C.; MEDEIROS, F. H. V. de; ARRUDA, J. H. Experimentos cooperativos de controle biológico de Sclerotinia sclerotiorum na cultura da soja: resultados sumarizados da safra 2020/2021. Londrina: Embrapa Soja, 2021. 12 p. (Embrapa Soja. Circular técnica, 177).| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Soja. |
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| 16. | | MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; JACCOUD FILHO, D. de S.; VENANCIO, W. S.; BRUSTOLIN, R.; CARNEIRO, L. C.; NUNES JUNIOR, J.; LOBO JUNIOR, M.; JULIATTI, F. C.; MEDEIROS, F. H. V. de; PIZOLOTTO, C. A.; SOUZA, T. P. de; OLIVEIRA, M. C. N. de. Experimentos cooperativos de controle biológico de Sclerotinia sclerotiorum na cultura da soja: resultados sumarizados da safra 2021/2022. Londrina: Embrapa Soja, 2022. 11 p. (Embrapa Soja. Circular técnica, 186).| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Soja. |
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| 17. | | MEYER, M. C.; GODOY, C. V.; UTIAMADA, C. M.; CAMPOS, H. D.; MEDEIROS, F. H. V. de; OLIVEIRA, M. C. N. de; MAZARO, S. M.; SENGER, M.; BONANI, J. C.; CARVALHO, A. G. de; BRUSTOLIN, R.; NUNES JUNIOR, J.; DIAS, A. R.; FANTIN, L. H.; GOUSSAIN JUNIOR, M. M.; GOMES, L. M. M. R.; FARIAS, A. de; CHAVES, I. C. P. de V.; MARTINS, M. C.; BELUFI, L. M. de R.; KONAGESKI, F. T.; KONAGESKI, T. F.; STEFANELO, M. S.; GRIGOLLI, J. F. J. Avaliação da associação de fungicidas químicos e biológicos no controle de doenças foliares da soja, safra 2022/2023: resultados sumarizados da rede de experimentos cooperativos. Londrina: Embrapa Soja, 2023. 14 p. (Embrapa Soja. Circular Técnica, 198).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 17 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
15/10/2020 |
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Data da última atualização: |
15/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W. |
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Afiliação: |
LARISSA CASTRO DE REZANDE, UFLA; ANDRÉ LUIZ DE ANDRADE CARVALHO, UFLA; LÚCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; LUCAS GUEDES SILVA, FCA-UNESP; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLÁVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFLA; GABRIEL MOURA MASCARIN, CNPMA; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020. |
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ISSN: |
1573-0972 |
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DOI: |
https://doi.org/10.1007/s11274-020-02882-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum. MenosAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these opt... Mostrar Tudo |
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Palavras-Chave: |
Shite mold; Submerged spores. |
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Thesagro: |
Fermentação; Fungo Para Controle Biológico; Trichoderma. |
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Thesaurus NAL: |
Biological control; Biological control agents; Bioreactors; Experimental design; Mass rearing; Molds (fungi); Solid state fermentation. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03117naa a2200397 a 4500
001 2125536
005 2020-10-15
008 2020 bl uuuu u00u1 u #d
022 $a1573-0972
024 7 $ahttps://doi.org/10.1007/s11274-020-02882-7$2DOI
100 1 $aREZENDE, L. C. de
245 $aOptimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum.$h[electronic resource]
260 $c2020
520 $aAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum.
650 $aBiological control
650 $aBiological control agents
650 $aBioreactors
650 $aExperimental design
650 $aMass rearing
650 $aMolds (fungi)
650 $aSolid state fermentation
650 $aFermentação
650 $aFungo Para Controle Biológico
650 $aTrichoderma
653 $aShite mold
653 $aSubmerged spores
700 1 $aCARVALHO, A. L. de A.
700 1 $aCOSTA, L. B.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aSILVA, L. G.
700 1 $aPINTO. Z. V.
700 1 $aMORANDI, M. A. B.
700 1 $aMEDEIROS, F. H. V. de
700 1 $aMASCARIN, G. M.
700 1 $aBETTIOL, W.
773 $tWorld Journal of Microbiology & Biotechnology$gv. 36, n. 8, article 113, 2020.
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